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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

C19orf36 Polyclonal antibody

C19orf36 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25650-1-AP

Synonyms

IZUMO4, Izumo sperm-egg fusion protein 4

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using C19orf36 Polyclonal antibody (25650-1-AP)

    WB analysis of mouse testis using 25650-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 25650-1-AP (C19orf36 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using C19orf36 Polyclonal antibody (25650-1-AP)

    WB analysis of HepG2 using 25650-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25650-1-AP (C19orf36 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using C19orf36 Polyclonal antibody (25650-1-AP)

    IHC staining of mouse testis using 25650-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25650-1-AP (C19orf36 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using C19orf36 Polyclonal antibody (25650-1-AP)

    IHC staining of mouse testis using 25650-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25650-1-AP (C19orf36 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using C19orf36 Polyclonal antibody (25650-1-AP)

    IHC staining of human testis using 25650-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25650-1-AP (C19orf36 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using C19orf36 Polyclonal antibody (25650-1-AP)

    IHC staining of human testis using 25650-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25650-1-AP (C19orf36 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using C19orf36 Polyclonal antibody (25650-1-AP)

    IF Staining of HepG2 using 25650-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using C19orf36 antibody (25650-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inmouse testis tissue, HepG2 cells
Positive IHC detected inmouse testis tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25650-1-AP targets C19orf36 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitybovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C19orf36 fusion protein Ag20434 相同性解析による交差性が予測される生物種
Full Name chromosome 19 open reading frame 36
Calculated molecular weight 232 aa, 27 kDa
Observed molecular weight 27-30 kDa
GenBank accession numberBC014609
Gene symbol C19orf36
Gene ID (NCBI) 113177
RRIDAB_2880175
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IZUMO4, also named as C19orf36, is sperm 22 kDa protein c113. It has 4 isoforms with MW 17-27 kDa.

Protocols

Product Specific Protocols
WB protocol for C19orf36 antibody 25650-1-APDownload protocol
IHC protocol for C19orf36 antibody 25650-1-APDownload protocol
IF protocol for C19orf36 antibody 25650-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
bovineIF,WB

Int J Biol Macromol

TMT-based quantitative N-glycoproteomic analysis reveals glycoprotein protection can improve the quality of frozen bovine sperm.

Authors - Na Wang
View Article