C19orf50 Polyclonal antibody
C19orf50 Polyclonal Antibody for WB, IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, Mouse
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 20492-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A549 cells, Calu-3 cells, NCI-H1299 cells, human placenta tissue |
Positive IHC detected in | mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | A549 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
20492-1-AP targets C19orf50 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, Mouse samples.
Tested Reactivity | human, Mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | C19orf50 fusion protein Ag13743 相同性解析による交差性が予測される生物種 |
Full Name | chromosome 19 open reading frame 50 |
Calculated molecular weight | 176 aa, 20 kDa |
Observed molecular weight | 28 kDa |
GenBank accession number | BC001080 |
Gene symbol | C19orf50 |
Gene ID (NCBI) | 79036 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
C19orf50, also known as KXD1 (KxDL motif-containing protein 1), a human uncharacterized coiled-coil protein, is a BLOS1-interacting protein. C19orf50 has been shown recently to interact with BLOC-1 subunits and may play a role in lysosome movement and localization at the cell periphery. C19orf50 was evaluated in NSCLC and LUAD (PMID: 33936200, 22554196).
Protocols
Product Specific Protocols | |
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WB protocol for C19orf50 antibody 20492-1-AP | Download protocol |
IHC protocol for C19orf50 antibody 20492-1-AP | Download protocol |
IF protocol for C19orf50 antibody 20492-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |