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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

C19orf50 Polyclonal antibody

C19orf50 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20492-1-AP

Synonyms

KXD1, UPF0459 protein C19orf50, MSTP096, MST096, KxDL motif-containing protein 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C19orf50 Polyclonal antibody (20492-1-AP)

    WB analysis using 20492-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20492-1-AP (C19orf50 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using C19orf50 Polyclonal antibody (20492-1-AP)

    IHC staining of mouse cerebellum using 20492-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 20492-1-AP (C19orf50 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using C19orf50 Polyclonal antibody (20492-1-AP)

    IHC staining of mouse cerebellum using 20492-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 20492-1-AP (C19orf50 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using C19orf50 Polyclonal antibody (20492-1-AP)

    IF Staining of A549 using 20492-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using C19orf50 antibody (20492-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive WB detected inA549 cells, Calu-3 cells, NCI-H1299 cells, human placenta tissue
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20492-1-AP targets C19orf50 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C19orf50 fusion protein Ag13743 相同性解析による交差性が予測される生物種
Full Name chromosome 19 open reading frame 50
Calculated molecular weight 176 aa, 20 kDa
Observed molecular weight28 kDa
GenBank accession numberBC001080
Gene symbol C19orf50
Gene ID (NCBI) 79036
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

C19orf50, also known as KXD1 (KxDL motif-containing protein 1), a human uncharacterized coiled-coil protein, is a BLOS1-interacting protein. C19orf50 has been shown recently to interact with BLOC-1 subunits and may play a role in lysosome movement and localization at the cell periphery. C19orf50 was evaluated in NSCLC and LUAD (PMID: 33936200, 22554196).

Protocols

Product Specific Protocols
WB protocol for C19orf50 antibody 20492-1-APDownload protocol
IHC protocol for C19orf50 antibody 20492-1-APDownload protocol
IF protocol for C19orf50 antibody 20492-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols