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  • KD/KO Validated

C19orf52 Polyclonal antibody

C19orf52 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 25652-1-AP

Synonyms

TIMM29, TIM29, Mitochondrial import inner membrane translocase subunit Tim29


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Tested Applications

Positive WB detected inmouse skeletal muscle tissue
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25652-1-AP targets C19orf52 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C19orf52 fusion protein Ag22251 相同性解析による交差性が予測される生物種
Full Name chromosome 19 open reading frame 52
Calculated molecular weight 260 aa, 29 kDa
Observed molecular weight 29-32 kDa
GenBank accession numberBC005075
Gene symbol C19orf52
Gene ID (NCBI) 90580
RRIDAB_2880177
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for C19orf52 antibody 25652-1-APDownload protocol
IHC protocol for C19orf52 antibody 25652-1-APDownload protocol
IP protocol for C19orf52 antibody 25652-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Metab

Acylglycerol Kinase Maintains Metabolic State and Immune Responses of CD8+ T Cells.

Authors - Zhilin Hu
humanWB

Mol Cell

Acylglycerol Kinase Mutated in Sengers Syndrome Is a Subunit of the TIM22 Protein Translocase in Mitochondria.

Authors - Milena Vukotic
humanWB

J Med Virol

TIM22 and TIM29 inhibit HBV replication by up-regulating SRSF1 expression

Authors - Lin Guo
  • KD Validated
humanWB

J Mol Biol

Mitochondrial Oxidative Stress Induces Rapid Intermembrane Space/Matrix Translocation of Apurinic/Apyrimidinic Endonuclease 1 Protein through TIM23 Complex.

Authors - Arianna Barchiesi
humanWB

Mol Biol Cell

Ovarian carcinoma immunoreactive antigen-like protein 2 (OCIAD2) is a novel complex III-specific assembly factor in mitochondria.

Authors - Katarzyna Justyna Chojnacka
humanWB

FEBS Lett

TIM29 is a subunit of the human carrier translocase required for protein transport.

Authors - Sylvie Callegari
  • KD Validated