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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C19orf57 Polyclonal antibody (25050-1-AP)

    WB analysis using 25050-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25050-1-AP (C19orf57 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using C19orf57 Polyclonal antibody (25050-1-AP)

    WB analysis of Jurkat using 25050-1-AP

    WB result of C19orf57 antibody (25050-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-C19orf57 transfected Jurkat cells.

  • Western Blot (WB) analysis of PC-3 cells using C19orf57 Polyclonal antibody (25050-1-AP)

    WB analysis of PC-3 using 25050-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 25050-1-AP (C19orf57 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A2780 cells using C19orf57 Polyclonal antibody (25050-1-AP)

    WB analysis of A2780 using 25050-1-AP

    A2780 cells were subjected to SDS PAGE followed by western blot with 25050-1-AP (C19orf57 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using C19orf57 Polyclonal antibody (25050-1-AP)

    WB analysis of A431 using 25050-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 25050-1-AP (C19orf57 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using C19orf57 Polyclonal antibody (25050-1-AP)

    WB analysis of K-562 using 25050-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 25050-1-AP (C19orf57 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using C19orf57 Polyclonal antibody (25050-1-AP)

    IHC staining of mouse testis using 25050-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25050-1-AP (C19orf57 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using C19orf57 Polyclonal antibody (25050-1-AP)

    IF Staining of A431 using 25050-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using C19orf57 antibody (25050-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

  • Featured Product
  • KD/KO Validated

C19orf57 Polyclonal antibody

C19orf57 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 25050-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Pre-T/NK cell-associated protein 3B3, BRME1, Break repair meiotic recombinase recruitment factor 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, K-562 cells, PC-3 cells, A431 cells, A2780 cells, MOLT-4 cells, mouse testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25050-1-AP targets C19orf57 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C19orf57 fusion protein Ag21745 相同性解析による交差性が予測される生物種
Full Name chromosome 19 open reading frame 57
Calculated molecular weight 668 aa, 70 kDa
Observed molecular weight 60-100 kDa
GenBank accession numberBC119719
Gene Symbol C19orf57
Gene ID (NCBI) 79173
RRIDAB_2879871
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ0VDD7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for C19orf57 antibody 25050-1-APDownload protocol
IHC protocol for C19orf57 antibody 25050-1-APDownload protocol
IF protocol for C19orf57 antibody 25050-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols