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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

C19orf61 Polyclonal antibody

C19orf61 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 24797-1-AP

Synonyms

SMG9, Protein SMG9, Nonsense-mediated mRNA decay factor SMG9, F17127_1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using C19orf61 Polyclonal antibody (24797-1-AP)

    WB analysis of HeLa using 24797-1-AP

    WB result of C19orf61 antibody (24797-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-C19orf61 transfected HeLa cells.

  • Western Blot (WB) analysis of HeLa cells using C19orf61 Polyclonal antibody (24797-1-AP)

    WB analysis of HeLa using 24797-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24797-1-AP (C19orf61 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using C19orf61 Polyclonal antibody (24797-1-AP)

    IP experiment of HeLa using 24797-1-AP

    IP result of anti-C19orf61 (IP:24797-1-AP, 3ug; Detection:24797-1-AP 1:500) with HeLa cells lysate 2000ug.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using C19orf61 Polyclonal antibody (24797-1-AP)

    IF Staining of U2OS using 24797-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using C19orf61 antibody (24797-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24797-1-AP targets C19orf61 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C19orf61 fusion protein Ag14965 相同性解析による交差性が予測される生物種
Full Name chromosome 19 open reading frame 61
Calculated molecular weight 520 aa, 58 kDa
Observed molecular weight 57-65 kDa
GenBank accession numberBC008869
Gene symbol C19orf61
Gene ID (NCBI) 56006
RRIDAB_2879731
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for C19orf61 antibody 24797-1-APDownload protocol
IF protocol for C19orf61 antibody 24797-1-APDownload protocol
IP protocol for C19orf61 antibody 24797-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Heliyon

SMG9 is a novel prognostic-related biomarker in glioma correlating with ferroptosis and immune infiltrates

Authors - Yong Dai
  • KD Validated
View Article