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Validation Data Gallery

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  • Western Blot (WB) analysis of T-47D cells using C1GALT1 Polyclonal antibody (27569-1-AP)

    WB analysis of T-47D using 27569-1-AP

    T-47D cells were subjected to SDS PAGE followed by western blot with 27569-1-AP (C1GALT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using C1GALT1 Polyclonal antibody (27569-1-AP)

    WB analysis using 27569-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27569-1-AP (C1GALT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using C1GALT1 Polyclonal antibody (27569-1-AP)

    WB analysis of HepG2 using 27569-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 27569-1-AP (C1GALT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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C1GALT1 Polyclonal antibody

C1GALT1 Polyclonal Antibody for WB, ELISA
Cat No. 27569-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

B3Gal T8, Beta 1,3 galactosyltransferase, C1GALT, Core 1 beta3 Gal T1, Core 1 O glycan T synthase

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMKN-45 cells, HepG2 cells, T-47D cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27569-1-AP targets C1GALT1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C1GALT1 fusion protein Ag26144 相同性解析による交差性が予測される生物種
Full Name core 1 synthase, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase, 1
Calculated molecular weight 42 kDa
Observed molecular weight 42-45 kDa
GenBank accession numberNM_020156
Gene Symbol C1GALT1
Gene ID (NCBI) 56913
RRIDAB_2880912
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NS00
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

C1GALT1 (Glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1), also known as B3Gal-T8, catalyzes the transfer of galactose (Gal) from UDP-Gal to N-acetylgalactosamine (GalNAc) O-linked esters of threonine and serine residues of target proteins, including IgA1, to form the T antigen (PMID: 28209808). C1GalT1 is overexpressed in many common cancers includingcolon, breast, gastric, lung, head and neck, pancreatic, esophageal, prostate, and hepatocellular cancer (PMID: 34944925). C1GALT1 protein contains 363 amino acids and is a typeII transmembrane protein. Rate C1GalT1 is an 84/86KDa disulfide-bonded homodimer, but its active form is believed to be a 42/43 KDa monomeric protein (PMID: 34149900).

Protocols

Product Specific Protocols
WB protocol for C1GALT1 antibody 27569-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Int Immunopharmacol

The upregulation of miR-98-5p affects the glycosylation of IgA1 through cytokines in IgA nephropathy.

Authors - Di Liu
View Article
humanIHC

J Biomed Res

Genetic variants in C1GALT1 are associated with gastric cancer risk and immune infiltrations

Authors - Mengfan Guo
View Article