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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

C1QA Monoclonal antibody, PBS Only

C1QA Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B10E3

Cat no : 67063-1-PBS

Synonyms

Complement C1q A chain, 1B10E3

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using C1QA Monoclonal antibody (67063-1-Ig)

    WB analysis of human plasma using 67063-1-Ig (same clone as 67063-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 67063-1-Ig (C1QA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67063-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human blood using C1QA Monoclonal antibody (67063-1-Ig)

    WB analysis of human blood using 67063-1-Ig (same clone as 67063-1-PBS)

    human blood were subjected to SDS PAGE followed by western blot with 67063-1-Ig (C1QA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67063-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using C1QA Monoclonal antibody (67063-1-Ig)

    IHC staining of human liver using 67063-1-Ig (same clone as 67063-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67063-1-Ig (C1QA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67063-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using C1QA Monoclonal antibody (67063-1-Ig)

    IHC staining of human liver using 67063-1-Ig (same clone as 67063-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67063-1-Ig (C1QA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67063-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using C1QA Monoclonal antibody (67063-1-Ig)

    IF Staining of HepG2 using 67063-1-Ig (same clone as 67063-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using C1QA antibody (67063-1-Ig, Clone: 1B10E3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67063-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67063-1-PBS targets C1QA in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen C1QA fusion protein Ag28622 相同性解析による交差性が予測される生物種
Full Name complement component 1, q subcomponent, A chain
Calculated molecular weight 245 aa, 26 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC030153
Gene symbol C1QA
Gene ID (NCBI) 712
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The first component of complement, C1, is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. This antibody is raised against C1qA which is the A-chain polypeptide of human complement subcomponent C1q.