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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using C1QBP Monoclonal antibody (68084-1-Ig)

    WB analysis of HEK-293 using 68084-1-Ig (same clone as 68084-1-PBS)

    WB result of C1QBP antibody (68084-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-C1QBP transfected HEK-293 cells. This data was developed using the same antibody clone with 68084-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using C1QBP Monoclonal antibody (68084-1-Ig)

    WB analysis using 68084-1-Ig (same clone as 68084-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68084-1-Ig (C1QBP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68084-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using C1QBP Monoclonal antibody (68084-1-Ig)

    WB analysis of mouse brain using 68084-1-Ig (same clone as 68084-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 68084-1-Ig (C1QBP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68084-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using C1QBP Monoclonal antibody (68084-1-Ig)

    IHC staining of human tonsillitis using 68084-1-Ig (same clone as 68084-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68084-1-Ig (C1QBP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68084-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using C1QBP Monoclonal antibody (68084-1-Ig)

    IHC staining of human tonsillitis using 68084-1-Ig (same clone as 68084-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68084-1-Ig (C1QBP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68084-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using C1QBP Monoclonal antibody (68084-1-Ig)

    IHC staining of human liver cancer using 68084-1-Ig (same clone as 68084-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68084-1-Ig (C1QBP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68084-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using C1QBP Monoclonal antibody (68084-1-Ig)

    IHC staining of human liver cancer using 68084-1-Ig (same clone as 68084-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68084-1-Ig (C1QBP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68084-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using C1QBP Monoclonal antibody (68084-1-Ig)

    IF Staining of HeLa using 68084-1-Ig (same clone as 68084-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using C1QBP antibody (68084-1-Ig, Clone: 1F9B1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68084-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using C1QBP Monoclonal antibody (68084-1-Ig)

    FC experiment of HeLa using 68084-1-Ig (same clone as 68084-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human C1QBP (68084-1-Ig, Clone:1F9B1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68084-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

C1QBP Monoclonal antibody, PBS Only

C1QBP Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 68084-1-PBS
Clone No.1F9B1

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

gC1Q R, GC1QBP, GC1q R protein, Complement component 1 Q subcomponent-binding protein, mitochondrial, ASF/SF2-associated protein p32

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68084-1-PBS targets C1QBP in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen C1QBP fusion protein Ag19773 相同性解析による交差性が予測される生物種
Full Name complement component 1, q subcomponent binding protein
Calculated molecular weight 282 aa, 31 kDa
Observed molecular weight32 kDa
GenBank accession numberBC013731
Gene Symbol C1QBP
Gene ID (NCBI) 708
RRIDAB_2918821
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ07021
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

C1QBP, also named as gC1q receptor (gC1qR), p32, p33, and hyaluronan-binding protein 1 (HABP1), is a protein initially copurified with splicing factor SF2 (PMID: 1830244). The protein is synthesized as a pro-protein of 282 amino acids (aa) that is post-translationally processed by removal of the initial 73 aa to a mature protein of 209 aa (PMID: 8262387). C1QBP is an evolutionary conserved and ubiquitously expressed multifunctional protein and has been reported to be a predominantly mitochondrial matrix protein involved in inflammation and infection processes, mitochondrial ribosome biogenesis, regulation of apoptosis and nuclear transcription, and pre-mRNA splicing (PMID: 28942965).