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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

C1QBP Recombinant antibody, PBS Only

C1QBP Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

rat, mouse, human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3K20

Cat no : 80490-1-PBS

Synonyms

gC1Q R, GC1QBP, GC1q R protein, Complement component 1 Q subcomponent-binding protein, mitochondrial, ASF/SF2-associated protein p32

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C1QBP Recombinant antibody (80490-1-RR)

    WB analysis using 80490-1-RR (same clone as 80490-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80490-1-RR (C1QBP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80490-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using C1QBP Recombinant antibody (80490-1-RR)

    IHC staining of human tonsillitis using 80490-1-RR (same clone as 80490-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 80490-1-RR (C1QBP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80490-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using C1QBP Recombinant antibody (80490-1-RR)

    IHC staining of human tonsillitis using 80490-1-RR (same clone as 80490-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 80490-1-RR (C1QBP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80490-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using C1QBP Recombinant antibody (80490-1-RR)

    IF Staining of HeLa using 80490-1-RR (same clone as 80490-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using C1QBP antibody (80490-1-RR, Clone: 3K20 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red). This data was developed using the same antibody clone with 80490-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using C1QBP Recombinant antibody (80490-1-RR)

    FC experiment of HeLa using 80490-1-RR (same clone as 80490-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human C1QBP (80490-1-RR, Clone:3K20) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Rabbit IgG (KFA002), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 80490-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80490-1-RR

    Affinity and Kinetic Characterization of 80490-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80490-1-RR against Human C1QBP were performed. The affinity constant is 0.776 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80490-1-PBS targets C1QBP in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with rat, mouse, human samples.

Tested Reactivity rat, mouse, human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen C1QBP fusion protein Ag19773 相同性解析による交差性が予測される生物種
Full Name complement component 1, q subcomponent binding protein
Calculated molecular weight 282 aa, 31 kDa
Observed molecular weight32 kDa
GenBank accession numberBC013731
Gene symbol C1QBP
Gene ID (NCBI) 708
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

C1QBP, also named as gC1q receptor (gC1qR), p32, p33, and hyaluronan-binding protein 1 (HABP1), is a protein initially copurified with splicing factor SF2 (PMID: 1830244). The protein is synthesized as a pro-protein of 282 amino acids (aa) that is post-translationally processed by removal of the initial 73 aa to a mature protein of 209 aa (PMID: 8262387). C1QBP is an evolutionary conserved and ubiquitously expressed multifunctional protein and has been reported to be a predominantly mitochondrial matrix protein involved in inflammation and infection processes, mitochondrial ribosome biogenesis, regulation of apoptosis and nuclear transcription, and pre-mRNA splicing (PMID: 28942965).