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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

C1QL1 Polyclonal antibody

C1QL1 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 15757-1-AP

Synonyms

CRF, Complement component 1 Q subcomponent-like 1, C1QRF, C1q-related factor, C1q/TNF-related protein 14

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C1QL1 Polyclonal antibody (15757-1-AP)

    WB analysis using 15757-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15757-1-AP (C1QL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using C1QL1 Polyclonal antibody (15757-1-AP)

    IHC staining of mouse cerebellum using 15757-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15757-1-AP (C1QL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using C1QL1 Polyclonal antibody (15757-1-AP)

    IHC staining of mouse cerebellum using 15757-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15757-1-AP (C1QL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using C1QL1 Polyclonal antibody (15757-1-AP)

    IF Staining of mouse cerebellum using 15757-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using C1QL1 antibody (15757-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse cerebellum tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15757-1-AP targets C1QL1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C1QL1 fusion protein Ag8348 相同性解析による交差性が予測される生物種
Full Name complement component 1, q subcomponent-like 1
Calculated molecular weight 258 aa, 26 kDa
Observed molecular weight30 kDa
GenBank accession numberBC008798
Gene symbol C1QL1
Gene ID (NCBI) 10882
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

C1QL1 (complement component 1, q subcomponent-like 1) is one of the C1q/tumor-necrosis-factor-related protein (CTRP) family members. C1QL1 is synthesized and secreted mainly by the brain and adipose tissues. It is implicated in synapse formation or elimination in cultured hippocampal neurons in vitro.

Protocols

Product Specific Protocols
WB protocol for C1QL1 antibody 15757-1-APDownload protocol
IHC protocol for C1QL1 antibody 15757-1-APDownload protocol
IF protocol for C1QL1 antibody 15757-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols