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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CTRP4 Polyclonal antibody

CTRP4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14023-1-AP

Synonyms

C1QTNF4, Complement C1q tumor necrosis factor-related protein 4, C1q/TNF-related protein 4

Validation Data Gallery

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  • Western Blot (WB) analysis of BxPC-3 cells using CTRP4 Polyclonal antibody (14023-1-AP)

    WB analysis of BxPC-3 using 14023-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14023-1-AP (CTRP4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using CTRP4 Polyclonal antibody (14023-1-AP)

    IHC staining of human brain using 14023-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 14023-1-AP (CTRP4 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using CTRP4 Polyclonal antibody (14023-1-AP)

    IHC staining of human brain using 14023-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 14023-1-AP (CTRP4 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of BxPC-3 cells using CTRP4 Polyclonal antibody (14023-1-AP)

    IF Staining of BxPC-3 using 14023-1-AP

    Immunofluorescent analysis of BxPC-3 cells using 14023-1-AP (CTRP4 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inBxPC-3 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inBxPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

14023-1-AP targets CTRP4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CTRP4 fusion protein Ag5147 相同性解析による交差性が予測される生物種
Full Name C1q and tumor necrosis factor related protein 4
Calculated molecular weight 329 aa, 35 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC035628
Gene symbol CTRP4
Gene ID (NCBI) 114900
RRIDAB_10644162
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CTRP4 antibody 14023-1-APDownload protocol
IHC protocol for CTRP4 antibody 14023-1-APDownload protocol
IF protocol for CTRP4 antibody 14023-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Ann Transl Med

C1Q/TNF-related protein 4 expression correlates with herpes simplex encephalitis progression.

Authors - Wangshu Xu
View Article