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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C1RL Polyclonal antibody (24820-1-AP)

    WB analysis using 24820-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24820-1-AP (C1RL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using C1RL Polyclonal antibody (24820-1-AP)

    IF Staining of U2OS using 24820-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using C1RL antibody (24820-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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C1RL Polyclonal antibody

C1RL Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 24820-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

C1r like protein, C1r LP, C1RL, C1RL1, C1RLP, CLSPa

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman plasma
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24820-1-AP targets C1RL in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C1RL fusion protein Ag20324 相同性解析による交差性が予測される生物種
Full Name complement component 1, r subcomponent-like
Calculated molecular weight 487 aa, 53 kDa
Observed molecular weight70 kDa
GenBank accession numberBC156780
Gene Symbol C1RL
Gene ID (NCBI) 51279
RRIDAB_3085758
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NZP8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

C1r-like serine protease analog protein (CLSPa), also named as CIRL contains a CUB domain and a serine protease domain, possessing characteristic catalytic triad. C1RL is upregulated in glioblastoma (GBM) and has been proved to be a prognostic marker in hepatocellular carcinoma and renal cell cancer (PMID: 32993564). C1RL has a calculated molecular weight of 53 kDa, and this antibody can detect a band at 70 kDa due to possible N-glycosylation (PMID: 15358180).

Protocols

Product Specific Protocols
WB protocol for C1RL antibody 24820-1-APDownload protocol
IF protocol for C1RL antibody 24820-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols