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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

C1orf116 Polyclonal antibody

C1orf116 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14888-1-AP

Synonyms

Specifically androgen-regulated gene protein, SARG

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of PC-3 cells using C1orf116 Polyclonal antibody (14888-1-AP)

    WB analysis of PC-3 using 14888-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 14888-1-AP (C1orf116 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using C1orf116 Polyclonal antibody (14888-1-AP)

    WB analysis of mouse testis using 14888-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 14888-1-AP (C1orf116 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brown disease using C1orf116 Polyclonal antibody (14888-1-AP)

    IHC staining of human brown disease using 14888-1-AP

    Immunohistochemical analysis of paraffin-embedded human brown disease slide using 14888-1-AP (C1orf116 antibody) at dilution of 1:160 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach tissue using C1orf116 Polyclonal antibody (14888-1-AP)

    IHC staining of human stomach using 14888-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 14888-1-AP (C1orf116 antibody) at dilution of 1:160 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inPC-3 cells, mouse testis tissue
Positive IHC detected inhuman stomach tissue, human brown disease
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14888-1-AP targets C1orf116 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C1orf116 fusion protein Ag6675 相同性解析による交差性が予測される生物種
Full Name chromosome 1 open reading frame 116
Calculated molecular weight 64 kDa
Observed molecular weight 64 kDa, 38 kDa
GenBank accession numberBC000765
Gene symbol C1orf116
Gene ID (NCBI) 79098
RRIDAB_2228225
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Specifically androgen-regulated gene protein (SARG), encoded by C1orf116, is a 601-aa protein considered putative androgen-specific receptor. SARG mRNA is highly expressed in prostate and can be up-regulated by androgens, but not by glucocorticoids. Little is known about the function of SARG to date. Two isoforms of SARG are produced by alternative splicing encoding 64 kDa and 38 kDa proteins, and both isoforms can be recognized by this antibody 14888-1-AP.

Protocols

Product Specific Protocols
WB protocol for C1orf116 antibody 14888-1-APDownload protocol
IHC protocol for C1orf116 antibody 14888-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Front Oncol

The Specifically Androgen-Regulated Gene (SARG) Promotes Papillary Thyroid Carcinoma (PTC) Lymphatic Metastasis Through Vascular Endothelial Growth Factor C (VEGF-C) and VEGF Receptor 3 (VEGFR-3) Axis.

Authors - Shuai-Jun Xu
View Article