Validation Data Gallery
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Tested Applications
| Positive WB detected in | A431 cells, COLO 320 cells, PC-3 cells |
| Positive IHC detected in | human placenta tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IF | See 1 publications below |
Product Information
25361-1-AP targets COA7 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | COA7 fusion protein Ag19734 相同性解析による交差性が予測される生物種 |
| Full Name | chromosome 1 open reading frame 163 |
| Calculated molecular weight | 231 aa, 26 kDa |
| Observed molecular weight | 25-28 kDa |
| GenBank accession number | BC015313 |
| Gene Symbol | COA7 |
| Gene ID (NCBI) | 65260 |
| RRID | AB_2880043 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96BR5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Cytochrome c oxidase assembly factor 7 (COA7) is a 231 aa long mitochondrial protein present in animals, containing five Sel1-like tetratricopeptide repeat sequences, which are likely to interact with partner proteins (PMID: 30885959). COA7 is a metazoan-specific assembly factor, the absence or mutation of which in humans accompanies complex IV assembly defects and neurological conditions (PMID: 35210360). COA7 has a molecular weight of ~25 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for COA7 antibody 25361-1-AP | Download protocol |
| IHC protocol for COA7 antibody 25361-1-AP | Download protocol |
| IF protocol for COA7 antibody 25361-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Med Genet COA7 (C1orf163/RESA1) mutations associated with mitochondrial leukoencephalopathy and cytochrome c oxidase deficiency. | ||
Mol Biol Cell Discovery of bactericides as an acute mitochondrial membrane damage inducer. | ||
iScience Bisbiguanide analogs induce mitochondrial stress to inhibit lung cancer cell invasion |