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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

C1orf83 Polyclonal antibody

C1orf83 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20940-1-AP

Synonyms

Transcription elongation factor A N-terminal and central domain-containing protein 2, TCEANC2, RP4 758J24.3, FLJ39169, FLJ32112

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C1orf83 Polyclonal antibody (20940-1-AP)

    WB analysis using 20940-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20940-1-AP (C1orf83 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using C1orf83 Polyclonal antibody (20940-1-AP)

    IHC staining of human brain using 20940-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 20940-1-AP (C1orf83 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using C1orf83 Polyclonal antibody (20940-1-AP)

    IHC staining of human brain using 20940-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 20940-1-AP (C1orf83 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using C1orf83 Polyclonal antibody (20940-1-AP)

    IHC staining of mouse brain using 20940-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20940-1-AP (C1orf83 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using C1orf83 Polyclonal antibody (20940-1-AP)

    IHC staining of mouse brain using 20940-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20940-1-AP (C1orf83 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using C1orf83 Polyclonal antibody (20940-1-AP)

    IHC staining of mouse cerebellum using 20940-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 20940-1-AP (C1orf83 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using C1orf83 Polyclonal antibody (20940-1-AP)

    IHC staining of mouse cerebellum using 20940-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 20940-1-AP (C1orf83 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using C1orf83 Polyclonal antibody (20940-1-AP)

    IHC staining of human breast cancer using 20940-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 20940-1-AP (C1orf83 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using C1orf83 Polyclonal antibody (20940-1-AP)

    IHC staining of human breast cancer using 20940-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 20940-1-AP (C1orf83 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using C1orf83 Polyclonal antibody (20940-1-AP)

    IF Staining of HeLa using 20940-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 20940-1-AP (C1orf83 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells, K-562 cells
Positive IHC detected inmouse cerebellum tissue, human brain tissue, human breast cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20940-1-AP targets C1orf83 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C1orf83 fusion protein Ag15040 相同性解析による交差性が予測される生物種
Full Name chromosome 1 open reading frame 83
Calculated molecular weight 208 aa, 23 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC035374
Gene symbol C1orf83
Gene ID (NCBI) 127428
RRIDAB_10860108
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Chromosome 1 open reading frame 83 (C1orf83) is also called TCEANC2, a little-studied transcription elongation factor. The rs10788972 variant of the TCEANC2 gene in the PARK10 region is closely associated with sporadic PD in the American population, while both rs10788972 and rs12046178 variants are not associated with sporadic PD in the Chinese Han population (PMID: 25663231; PMID: 26432391).

Protocols

Product Specific Protocols
WB protocol for C1orf83 antibody 20940-1-APDownload protocol
IHC protocol for C1orf83 antibody 20940-1-APDownload protocol
IF protocol for C1orf83 antibody 20940-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols