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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

C1orf83 Recombinant antibody, PBS Only (Detector)

C1orf83 Recombinant Antibody for IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240678D10

Cat no : 83626-3-PBS

Synonyms

Transcription elongation factor A N-terminal and central domain-containing protein 2, TCEANC2, RP4 758J24.3, FLJ39169, FLJ32112

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using C1orf83 Recombinant antibody (83626-3-RR)

    IHC staining of mouse cerebellum using 83626-3-RR (same clone as 83626-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83626-3-RR (C1orf83 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83626-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using C1orf83 Recombinant antibody (83626-3-RR)

    IHC staining of mouse cerebellum using 83626-3-RR (same clone as 83626-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83626-3-RR (C1orf83 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83626-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using C1orf83 Recombinant antibody (83626-3-RR)

    IF Staining of HEK-293 using 83626-3-RR (same clone as 83626-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using C1orf83 antibody (83626-3-RR, Clone: 240678D10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83626-3-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U2OS cells using C1orf83 Recombinant antibody (83626-3-RR)

    FC experiment of U2OS using 83626-3-RR (same clone as 83626-3-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug C1orf83 Recombinant antibody (83626-3-RR, Clone:240678D10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83626-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00578-2

    Cytometric bead array standard curve of MP00578-2

    Cytometric bead array standard curve of MP00578-2, C1orf83 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83626-1-PBS. Detection antibody: 83626-3-PBS. Standard: Ag15040. Range: 0.78-100 ng/mL.

  • Affinity and Kinetic Characterization of 83626-3-RR

    Affinity and Kinetic Characterization of 83626-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83626-3-RR against Human C1orf83 were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83626-3-PBS targets C1orf83 as part of a matched antibody pair:

MP00578-2: 83626-1-PBS capture and 83626-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen C1orf83 fusion protein Ag15040 相同性解析による交差性が予測される生物種
Full Name chromosome 1 open reading frame 83
Calculated molecular weight 208 aa, 23 kDa
GenBank accession numberBC035374
Gene symbol C1orf83
Gene ID (NCBI) 127428
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.