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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

C1orf83 Recombinant antibody

C1orf83 Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240678D10

Cat no : 83626-3-RR

Synonyms

Transcription elongation factor A N-terminal and central domain-containing protein 2, TCEANC2, RP4 758J24.3, FLJ39169, FLJ32112

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using C1orf83 Recombinant antibody (83626-3-RR)

    IHC staining of mouse cerebellum using 83626-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83626-3-RR (C1orf83 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using C1orf83 Recombinant antibody (83626-3-RR)

    IHC staining of mouse cerebellum using 83626-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83626-3-RR (C1orf83 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using C1orf83 Recombinant antibody (83626-3-RR)

    IF Staining of HEK-293 using 83626-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using C1orf83 antibody (83626-3-RR, Clone: 240678D10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of U2OS cells using C1orf83 Recombinant antibody (83626-3-RR)

    FC experiment of U2OS using 83626-3-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug C1orf83 Recombinant antibody (83626-3-RR, Clone:240678D10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83626-3-RR

    Affinity and Kinetic Characterization of 83626-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83626-3-RR against Human C1orf83 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Positive FC (Intra) detected inU2OS cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83626-3-RR is C1orf83 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen C1orf83 fusion protein Ag15040 相同性解析による交差性が予測される生物種
Full Name chromosome 1 open reading frame 83
Calculated molecular weight 208 aa, 23 kDa
GenBank accession numberBC035374
Gene symbol C1orf83
Gene ID (NCBI) 127428
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

C1orf83, also known as TCEANC2, and the rs10788972 variant of the TCEANC2 gene in the PARK10 region were identified as being strongly associated with sporadic PD in the US population.

Protocols

Product Specific Protocols
IHC protocol for C1orf83 antibody 83626-3-RRDownload protocol
IF protocol for C1orf83 antibody 83626-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols