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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

C1orf83 Recombinant antibody

C1orf83 Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240678F1

Cat no : 83626-5-RR

Synonyms

Transcription elongation factor A N-terminal and central domain-containing protein 2, TCEANC2, RP4 758J24.3, FLJ39169, FLJ32112

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C1orf83 Recombinant antibody (83626-5-RR)

    WB analysis using 83626-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83626-5-RR (C1orf83 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using C1orf83 Recombinant antibody (83626-5-RR)

    IF Staining of U2OS using 83626-5-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using C1orf83 antibody (83626-5-RR, Clone: 240678F1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83626-5-RR

    Affinity and Kinetic Characterization of 83626-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83626-5-RR against Human C1orf83 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83626-5-RR is C1orf83 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen C1orf83 fusion protein Ag15040 相同性解析による交差性が予測される生物種
Full Name chromosome 1 open reading frame 83
Calculated molecular weight 208 aa, 23 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC035374
Gene symbol C1orf83
Gene ID (NCBI) 127428
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Chromosome 1 open reading frame 83 (C1orf83) is also called TCEANC2, a little-studied transcription elongation factor. The rs10788972 variant of the TCEANC2 gene in the PARK10 region is closely associated with sporadic PD in the American population, while both rs10788972 and rs12046178 variants are not associated with sporadic PD in the Chinese Han population (PMID: 25663231; PMID: 26432391).

Protocols

Product Specific Protocols
WB protocol for C1orf83 antibody 83626-5-RRDownload protocol
IF protocol for C1orf83 antibody 83626-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols