GID8 Polyclonal antibody
GID8 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 24479-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, K-562 cells, HeLa cells, Jurkat cells, mouse brain tissue, rat brain tissue, SW480 cells, HepG2 cells |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
The immunogen of 24479-1-AP is GID8 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GID8 fusion protein Ag19751 相同性解析による交差性が予測される生物種 |
| Full Name | chromosome 20 open reading frame 11 |
| Calculated molecular weight | 228 aa, 27 kDa |
| Observed molecular weight | 30 kDa |
| GenBank accession number | BC032120 |
| Gene symbol | GID8 |
| Gene ID (NCBI) | 54994 |
| RRID | AB_2879562 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GID8 antibody 24479-1-AP | Download protocol |
| IHC protocol for GID8 antibody 24479-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
PLoS Genet Role of Bicaudal C1 in renal gluconeogenesis and its novel interaction with the CTLH complex. | ||
Sci Rep Tagging enhances histochemical and biochemical detection of Ran Binding Protein 9 in vivo and reveals its interaction with Nucleolin. | ||
bioRxiv Genome-wide screen of Mycobacterium tuberculosis- infected macrophages identified the GID/CTLH complex as a determinant of intracellular bacterial growth | ||
Cancer Lett YTHDF1 regulates GID8-mediated glutamine metabolism to promote colorectal cancer progression in a m6A-dependent manner | ||
Mol Cell mTORC1-CTLH E3 ligase regulates the degradation of HMG-CoA synthase 1 through the Pro/N-degron pathway |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH H (Verified Customer) (09-27-2020) | GID8 was pulled down with MAEA-flag, so I could tell which band is GID8. But without IP, it would be difficult to tell which band is the GID8, because the on-target band signal is too weak, while off-target band signals are strong in HEK293T cells
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