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C20orf3/APMAP Monoclonal antibody

C20orf3/APMAP Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, rabbit

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1C6C2

Cat no : 67464-1-Ig

Synonyms

C20orf3, Protein BSCv, BSCv, APMAP, Adipocyte plasma membrane-associated protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C20orf3/APMAP Monoclonal antibody (67464-1-Ig)

    WB analysis using 67464-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67464-1-Ig (C20orf3/APMAP antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using C20orf3/APMAP Monoclonal antibody (67464-1-Ig)

    WB analysis using 67464-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67464-1-Ig (C20orf3/APMAP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using C20orf3/APMAP Monoclonal antibody (67464-1-Ig)

    WB analysis of HepG2 using 67464-1-Ig

    WB result of C20orf3/APMAP antibody (67464-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-C20orf3/APMAP transfected HepG2 cells.

  • Western Blot (WB) analysis of various lysates using C20orf3/APMAP Monoclonal antibody (67464-1-Ig)

    WB analysis using 67464-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67464-1-Ig (C20orf3/APMAP antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using C20orf3/APMAP Monoclonal antibody (67464-1-Ig)

    WB analysis using 67464-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67464-1-Ig (C20orf3/APMAP antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using C20orf3/APMAP Monoclonal antibody (67464-1-Ig)

    IHC staining of human prostate cancer using 67464-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67464-1-Ig (C20orf3/APMAP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using C20orf3/APMAP Monoclonal antibody (67464-1-Ig)

    IHC staining of human prostate cancer using 67464-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67464-1-Ig (C20orf3/APMAP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using C20orf3/APMAP Monoclonal antibody (67464-1-Ig)

    IHC staining of human lung cancer using 67464-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67464-1-Ig (C20orf3/APMAP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using C20orf3/APMAP Monoclonal antibody (67464-1-Ig)

    IHC staining of human lung cancer using 67464-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67464-1-Ig (C20orf3/APMAP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inJurkat cells, HSC-T6 cells, rabbit liver tissue, HepG2 cells, LNCaP cells, NIH/3T3 cells, human placenta tissue, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67464-1-Ig targets C20orf3/APMAP in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.

Tested Reactivity human, mouse, rat, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen C20orf3/APMAP fusion protein Ag29996 相同性解析による交差性が予測される生物種
Full Name chromosome 20 open reading frame 3
Calculated molecular weight 416 aa, 46 kDa
Observed molecular weight 42-46 kDa
GenBank accession numberBC003501
Gene symbol APMAP
Gene ID (NCBI) 57136
RRIDAB_2882693
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for C20orf3/APMAP antibody 67464-1-IgDownload protocol
IHC protocol for C20orf3/APMAP antibody 67464-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols