- Featured Product
- KD/KO Validated
C20orf3/APMAP Polyclonal antibody
C20orf3/APMAP Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF, IP, ELISA
Conjugate
Unconjugated
Cat no : 25953-1-AP
Synonyms
Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | HepG2 cells |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
25953-1-AP targets C20orf3/APMAP in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | C20orf3/APMAP fusion protein Ag23029 相同性解析による交差性が予測される生物種 |
| Full Name | chromosome 20 open reading frame 3 |
| Calculated molecular weight | 416 aa, 46 kDa |
| Observed molecular weight | 42-46 kDa |
| GenBank accession number | BC003501 |
| Gene symbol | APMAP |
| Gene ID (NCBI) | 57136 |
| RRID | AB_2880308 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for C20orf3/APMAP antibody 25953-1-AP | Download protocol |
| IHC protocol for C20orf3/APMAP antibody 25953-1-AP | Download protocol |
| IP protocol for C20orf3/APMAP antibody 25953-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Res Cholesterol induces epithelial-to-mesenchymal transition of prostate cancer cells by suppressing degradation of EGFR through APMAP.
| ||
J Nutr Biochem Thioredoxin domain containing 5 is involved in the hepatic storage of squalene into lipid droplets in a sex-specific way |
