TMEM230 Polyclonal antibody
TMEM230 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 21466-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, COLO 320 cells, HEK-293 cells, HeLa cells |
| Positive IHC detected in | human brain tissue, human colon cancer tissue, human liver cancer tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
The immunogen of 21466-1-AP is TMEM230 Fusion Protein expressed in E. coli.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TMEM230 fusion protein Ag15080 相同性解析による交差性が予測される生物種 |
| Full Name | chromosome 20 open reading frame 30 |
| Calculated molecular weight | 183 aa, 20 kDa |
| Observed molecular weight | 13 kDa |
| GenBank accession number | BC011990 |
| Gene symbol | TMEM230 |
| Gene ID (NCBI) | 29058 |
| RRID | AB_10791969 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The function of TMEM230/C20orf30 has not been widely studied, and is yet to be fully elucidated. TMEM230 is primarily a transmembrane protein of synaptic vesicles in neurons. Disease-linked TMEM230 mutants impair synaptic vesicle trafficking leading to Parkinson's disease. Two isoforms were produced by alternative splicing with predicted MW of 13 and 20 kDa. 13 kDa isoform accounts for >95% of the total protein isoforms(27270108).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TMEM230 antibody 21466-1-AP | Download protocol |
| IHC protocol for TMEM230 antibody 21466-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Molecular identification of a BAR domain-containing coat complex for endosomal recycling of transmembrane proteins. | ||
J Alzheimers Dis TMEM230 Accumulation in Granulovacuolar Degeneration Bodies and Dystrophic Neurites of Alzheimer's Disease | ||
Exp Anim TMEM230 modulates seizure in epilepsy: evidence from temporal lobe epilepsy patients and mouse models | ||
Int J Mol Sci Transmembrane Protein TMEM230, Regulator of Glial Cell Vascular Mimicry and Endothelial Cell Angiogenesis in High-Grade Heterogeneous Infiltrating Gliomas and Glioblastoma |
