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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TMEM230 Monoclonal antibody

TMEM230 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

4D3A7

Cat no : 67247-1-Ig

Synonyms

C20orf30, dJ1116H23.2.1, HSPC274, TMEM230, UNQ2432/PRO4992

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TMEM230 Monoclonal antibody (67247-1-Ig)

    WB analysis using 67247-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67247-1-Ig (TMEM230 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TMEM230 Monoclonal antibody (67247-1-Ig)

    WB analysis of HeLa using 67247-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67247-1-Ig (C20orf30 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using TMEM230 Monoclonal antibody (67247-1-Ig)

    WB analysis of COLO 320 using 67247-1-Ig

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 67247-1-Ig (C20orf30 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Caco-2 cells using TMEM230 Monoclonal antibody (67247-1-Ig)

    WB analysis of Caco-2 using 67247-1-Ig

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 67247-1-Ig (C20orf30 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HT-29 cells using TMEM230 Monoclonal antibody (67247-1-Ig)

    WB analysis of HT-29 using 67247-1-Ig

    HT-29 cells were subjected to SDS PAGE followed by western blot with 67247-1-Ig (C20orf30 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using TMEM230 Monoclonal antibody (67247-1-Ig)

    WB analysis of A549 using 67247-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 67247-1-Ig (C20orf30 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using TMEM230 Monoclonal antibody (67247-1-Ig)

    WB analysis of MCF-7 using 67247-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67247-1-Ig (C20orf30 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHeLa cells, COLO 320 cells, Caco-2 cells, HT-29 cells, A549 cells, MCF-7 cells, HSC-T6 cells, NIH/3T3 cells, PC-12 cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67247-1-Ig targets TMEM230 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TMEM230 fusion protein Ag15080 相同性解析による交差性が予測される生物種
Full Name chromosome 20 open reading frame 30
Calculated molecular weight 183 aa, 20 kDa
Observed molecular weight 16 kDa
GenBank accession numberBC011990
Gene symbol TMEM230
Gene ID (NCBI) 29058
RRIDAB_2882524
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TMEM230 antibody 67247-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols