Validation Data Gallery
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Tested Applications
| Positive WB detected in | MCF-7 cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 2 publications below |
Product Information
16633-1-AP targets RTF2 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RTF2 fusion protein Ag9972 相同性解析による交差性が予測される生物種 |
| Full Name | chromosome 20 open reading frame 43 |
| Calculated molecular weight | 34 kDa |
| Observed molecular weight | 35-40 kDa |
| GenBank accession number | BC003359 |
| Gene Symbol | RTF2 |
| Gene ID (NCBI) | 51507 |
| RRID | AB_2256547 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BY42 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The function of C20orf43 remains largely unknown. Catalog#16633-1-AP is a rabbit polyclonal antibody raised against the full-length of human C20orf43. The MW of this protein is 40 kDa, and this antibody specially recognises the 40 kDa protein.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RTF2 antibody 16633-1-AP | Download protocol |
| IF protocol for RTF2 antibody 16633-1-AP | Download protocol |
| IP protocol for RTF2 antibody 16633-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Removal of RTF2 from Stalled Replisomes Promotes Maintenance of Genome Integrity.
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bioRxiv RTF2 controls replication repriming and ribonucleotide excision at the replisome
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