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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

C21orf2 Polyclonal antibody

C21orf2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 27609-1-AP

Synonyms

A2, C21orf HUMF09G8.5, C21orf2, YF5, YF5/A2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C21orf2 Polyclonal antibody (27609-1-AP)

    WB analysis using 27609-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 27609-1-AP (C21orf2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293T cells using C21orf2 Polyclonal antibody (27609-1-AP)

    WB analysis of HEK-293T using 27609-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 27609-1-AP (C21orf2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293T cells using C21orf2 Polyclonal antibody (27609-1-AP)

    WB analysis of HEK-293T using 27609-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 27609-1-AP (C21orf2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293T cells using human C21orf2 Polyclonal antibody (27609-1-AP)

    IP experiment of HEK-293T using 27609-1-AP

    IP result of anti-C21orf2 (IP:27609-1-AP, 4ug; Detection:27609-1-AP 1:3000) with HEK-293T cells lysate 1320 ug.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using C21orf2 Polyclonal antibody (27609-1-AP)

    IHC staining of human lung cancer using 27609-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27609-1-AP (C21orf2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using C21orf2 Polyclonal antibody (27609-1-AP)

    IHC staining of human lung cancer using 27609-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27609-1-AP (C21orf2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHEK-293T cells
Positive IP detected inHEK-293T cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27609-1-AP targets C21orf2 in WB, IHC, IP, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C21orf2 fusion protein Ag26470 相同性解析による交差性が予測される生物種
Full Name chromosome 21 open reading frame 2
Observed molecular weight 28-32 kDa
GenBank accession numberBC072012
Gene symbol C21orf2
Gene ID (NCBI) 755
RRIDAB_2880924
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for C21orf2 antibody 27609-1-APDownload protocol
IHC protocol for C21orf2 antibody 27609-1-APDownload protocol
IP protocol for C21orf2 antibody 27609-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

Life Sci Alliance

Functional characterization of C21ORF2 association with the NEK1 kinase mutated in human in diseases

Authors - Mateusz Gregorczyk
View Article
humanWB

Acta Neuropathol Commun

ALS-associated C21ORF2 variant disrupts DNA damage repair, mitochondrial metabolism, neuronal excitability and NEK1 levels in human motor neurons

Authors - Pavol Zelina
View Article