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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C21orf33 Polyclonal antibody (32198-1-AP)

    WB analysis using 32198-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32198-1-AP (C21orf33 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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C21orf33 Polyclonal antibody

C21orf33 Polyclonal Antibody for WB, ELISA
Cat No. 32198-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

GAL3A, Glutamine amidotransferase-like class 1 domain-containing protein 3, mitochondrial, GATD3A, GATD3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, Hela cells, HepG2 cells, K-562 cells, THP-1 cells, mouse liver tissue, rat liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32198-1-AP targets C21orf33 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C21orf33 fusion protein Ag37527 相同性解析による交差性が予測される生物種
Full Name chromosome 21 open reading frame 33
Calculated molecular weight 268 aa, 28 kDa
Observed molecular weight28 kDa
GenBank accession numberBC002370
Gene Symbol C21orf33
Gene ID (NCBI) 8209
RRIDAB_3670221
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP0DPI2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GATD3A is a member of glutamine amidotransferase-like class 1 domain-containing (GATD) family. GATD proteins classically generate ammonia by amidolysis from the glutamine amide nitrogen, transferring it to an acceptor substrate in a variety of anabolic reactions involving purine and pyrimidine synthesis (PMID: 9575335). GATD3A restricts the formation of AGEs in mitochondria and is a relevant target for diseases where AGE deposition is a pathological hallmark (PMID: 35307029).

Protocols

Product Specific Protocols
WB protocol for C21orf33 antibody 32198-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols