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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

C21orf45 Polyclonal antibody, PBS Only

C21orf45 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 25832-1-PBS

Synonyms

MIS18A, FASP1, FAPP1-associated protein 1, FAPP1 associated protein 1, C21orf46

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using C21orf45 Polyclonal antibody (25832-1-AP)

    WB analysis of HeLa using 25832-1-AP (same clone as 25832-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 25832-1-AP (C21orf45 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25832-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using C21orf45 Polyclonal antibody (25832-1-AP)

    IF Staining of HepG2 using 25832-1-AP (same clone as 25832-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using C21orf45 antibody (25832-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25832-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25832-1-PBS targets C21orf45 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C21orf45 fusion protein Ag22922 相同性解析による交差性が予測される生物種
Full Name chromosome 21 open reading frame 45
Calculated molecular weight 233 aa, 26 kDa
Observed molecular weight 105 kDa
GenBank accession numberBC042917
Gene symbol C21orf45
Gene ID (NCBI) 54069
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

C21orf45, also named as MIS18A, C21orf46 and FASP1, is required for recruitment of CENPA to centromeres and normal chromosome segregation during mitosis. C21orf45 has dimer(~52 kDa) and heterodimer(~105 kDa) forms.