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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

C2orf3 Polyclonal antibody

C2orf3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 26029-1-AP

Synonyms

C2orf3, DNABF, GCF, TCF 9, TCF9, Transcription factor 9

Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using C2orf3 Polyclonal antibody (26029-1-AP)

    WB analysis of PC-3 using 26029-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 26029-1-AP (C2orf3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using C2orf3 Polyclonal antibody (26029-1-AP)

    WB analysis of HeLa using 26029-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 26029-1-AP (C2orf3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using C2orf3 Polyclonal antibody (26029-1-AP)

    WB analysis of HepG2 using 26029-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 26029-1-AP (C2orf3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using C2orf3 Polyclonal antibody (26029-1-AP)

    WB analysis of HepG2 using 26029-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 26029-1-AP (C2orf3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inPC-3 cells, HeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26029-1-AP is C2orf3 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C2orf3 fusion protein Ag23315 相同性解析による交差性が予測される生物種
Full Name chromosome 2 open reading frame 3
Observed molecular weight 89 kDa
GenBank accession numberBC064559
Gene symbol C2orf3
Gene ID (NCBI) 6936
RRIDAB_2880343
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GC-rich sequence DNA-binding factor is a protein that in humans is encoded by the C2orf3 gene. C2orf3 belongs to the GCF family. It is a factor that represses transcription. It binds to the GC-rich sequences present in the epidermal growth factor receptor, beta-actin, and calcium-dependent protease promoters. The MW of this protein is 89 kDa, and this antibody specially recognises the 89 kDa protein.

Protocols

Product Specific Protocols
WB protocol for C2orf3 antibody 26029-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols