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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

C2orf47 Polyclonal antibody

C2orf47 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24930-1-AP

Synonyms

Matrix AAA peptidase-interacting protein 1, MAIP1, m-AAA protease-interacting protein 1, mitochondrial

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse kidney tissue using C2orf47 Polyclonal antibody (24930-1-AP)

    WB analysis of mouse kidney using 24930-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24930-1-AP (C2orf47 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using C2orf47 Polyclonal antibody (24930-1-AP)

    WB analysis of PC-3 using 24930-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 24930-1-AP (C2orf47 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using C2orf47 Polyclonal antibody (24930-1-AP)

    WB analysis of HeLa using 24930-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24930-1-AP (C2orf47 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using C2orf47 Polyclonal antibody (24930-1-AP)

    IHC staining of human kidney using 24930-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24930-1-AP (C2orf47 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using C2orf47 Polyclonal antibody (24930-1-AP)

    IHC staining of human kidney using 24930-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24930-1-AP (C2orf47 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse kidney tissue, HeLa cells, PC-3 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

24930-1-AP targets C2orf47 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C2orf47 fusion protein Ag21518 相同性解析による交差性が予測される生物種
Full Name chromosome 2 open reading frame 47
Calculated molecular weight 291 aa, 33 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC017959
Gene symbol C2orf47
Gene ID (NCBI) 79568
RRIDAB_2879804
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

C2orf47, also named matrix AAA peptidase interacting protein 1 (MAIP1), is a 291 amino-acid protein that localizes in the Mitochondrion. C2orf47 is involved in protecting EMRE, a component of the single calcium complex (MCU), from proteolysis by assisting membrane insertion.

Protocols

Product Specific Protocols
WB protocol for C2orf47 antibody 24930-1-APDownload protocol
IHC protocol for C2orf47 antibody 24930-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Nat Cell Biol

Small heat shock proteins operate as molecular chaperones in the mitochondrial intermembrane space

Authors - Elias Adriaenssens
View Article