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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

C2orf79/PTRHD1 Polyclonal antibody

C2orf79/PTRHD1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 25576-1-AP

Synonyms

PTRHD1, C2orf79, Putative peptidyl-tRNA hydrolase PTRHD1, Peptidyl-tRNA hydrolase domain-containing protein 1, EC:3.1.1.29

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using C2orf79/PTRHD1 Polyclonal antibody (25576-1-AP)

    WB analysis of HepG2 using 25576-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25576-1-AP (C2orf79/PTRHD1 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of COLO 320 cells using C2orf79/PTRHD1 Polyclonal antibody (25576-1-AP)

    IP experiment of COLO 320 using 25576-1-AP

    IP result of anti-C2orf79/PTRHD1 (IP:25576-1-AP, 4ug; Detection:25576-1-AP 1:500) with COLO 320 cells lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using C2orf79/PTRHD1 Polyclonal antibody (25576-1-AP)

    IHC staining of human colon cancer using 25576-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25576-1-AP (C2orf79/PTRHD1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using C2orf79/PTRHD1 Polyclonal antibody (25576-1-AP)

    IHC staining of human colon cancer using 25576-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25576-1-AP (C2orf79/PTRHD1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using C2orf79/PTRHD1 Polyclonal antibody (25576-1-AP)

    IF Staining of HepG2 using 25576-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 25576-1-AP (C2orf79/PTRHD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHepG2 cells
Positive IP detected inCOLO 320 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25576-1-AP targets C2orf79/PTRHD1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C2orf79/PTRHD1 fusion protein Ag22255 相同性解析による交差性が予測される生物種
Full Name chromosome 2 open reading frame 79
Calculated molecular weight 140 aa, 16 kDa
Observed molecular weight 13-16 kDa
GenBank accession numberBC073803
Gene symbol PTRHD1
Gene ID (NCBI) 391356
RRIDAB_2880136
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for C2orf79/PTRHD1 antibody 25576-1-APDownload protocol
IHC protocol for C2orf79/PTRHD1 antibody 25576-1-APDownload protocol
IF protocol for C2orf79/PTRHD1 antibody 25576-1-APDownload protocol
IP protocol for C2orf79/PTRHD1 antibody 25576-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols