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Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using C3/C3b/C3c Monoclonal antibody (66157-1-Ig)

    WB analysis of human plasma using 66157-1-Ig (same clone as 66157-1-PBS)

    human plasma was subjected to SDS PAGE followed by western blot with 66157-1-Ig (C3/C3b/C3c antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66157-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using C3/C3b/C3c Monoclonal antibody (66157-1-Ig)

    WB analysis using 66157-1-Ig (same clone as 66157-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66157-1-Ig (C3/C3b/C3c antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66157-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human blood using C3/C3b/C3c Monoclonal antibody (66157-1-Ig)

    WB analysis of human blood using 66157-1-Ig (same clone as 66157-1-PBS)

    human blood were subjected to SDS PAGE followed by western blot with 66157-1-Ig (C3/C3b/C3c antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66157-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using C3/C3b/C3c Monoclonal antibody (66157-1-Ig)

    IHC staining of human liver using 66157-1-Ig (same clone as 66157-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66157-1-Ig (C3/C3b/C3c antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66157-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using C3/C3b/C3c Monoclonal antibody (66157-1-Ig)

    IHC staining of human liver using 66157-1-Ig (same clone as 66157-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66157-1-Ig (C3/C3b/C3c antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66157-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using C3/C3b/C3c Monoclonal antibody (66157-1-Ig)

    IF Staining of HepG2 using 66157-1-Ig (same clone as 66157-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66157-1-Ig (C3/C3b/C3c antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66157-1-PBS in a different storage buffer formulation.

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C3/C3b/C3c Monoclonal antibody, PBS Only

C3/C3b/C3c Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66157-1-PBS
Clone No.2A6B3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

C3, C3/C3a, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, ASP, ARMD9

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66157-1-PBS targets C3/C3b/C3c in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen C3/C3b/C3c fusion protein Ag15955 相同性解析による交差性が予測される生物種
Full Name complement component 3
Calculated molecular weight 1663 aa, 187 kDa
Observed molecular weight 115 kDa
GenBank accession numberBC150179
Gene Symbol C3
Gene ID (NCBI) 718
RRIDAB_2881553
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP01024
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

The complement system is an important effector that bridges the innate and adaptive immune systems (PMID: 20010915). The third component of complement, C3, plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways (PMID: 11414361). Human C3, composed of α and β chains (115 and 75 kDa, respectively), is cleaved into C3a and C3b by C3 convertase. C3b is further cleaved into iC3b, C3c, C3dg and C3f. This antibody raised against 1314-1663 aa of human C3 protein recognizes the C3 alpha chain, C3b alpha' chain, and C3c alpha' chain fragment 2.