C4BPA Polyclonal antibody
C4BPA Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 11819-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | human blood tissue, human plasma tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 1 publications below |
Product Information
11819-1-AP targets C4BPA in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | C4BPA fusion protein Ag2387 相同性解析による交差性が予測される生物種 |
| Full Name | complement component 4 binding protein, alpha |
| Calculated molecular weight | 597 aa, 67 kDa |
| Observed molecular weight | 70 kDa |
| GenBank accession number | BC022312 |
| Gene symbol | C4BPA |
| Gene ID (NCBI) | 722 |
| RRID | AB_2877796 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
C4b-binding protein (C4BP) is a large oligomeric glycoprotein, present in plasma at a concentration of approximately 200 mg/L (PMID: 24218259). It is the main inhibitor of the classical complement and lectin pathway. C4BP is composed of two polypeptides (alpha and beta chains), which have molecular weights of 70 and 45 kDa, respectively (PMID: 7561113). This polyclonal antibody is raised against the C4BP alpha chain (C4BPA).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for C4BPA antibody 11819-1-AP | Download protocol |
| IHC protocol for C4BPA antibody 11819-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Oncotarget Hepatitis B virus X protein up-regulates C4b-binding protein α through activating transcription factor Sp1 in protection of hepatoma cells from complement attack. | ||
BMC Cancer The imbalance in the complement system and its possible physiological mechanisms in patients with lung cancer. | ||
World J Surg Oncol Proteomic analysis of small extracellular vesicles from the plasma of patients with hepatocellular carcinoma |
