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Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using C5 Monoclonal antibody (66634-1-Ig)

    WB analysis of human plasma using 66634-1-Ig (same clone as 66634-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 66634-1-Ig (C5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66634-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rabbit serum using C5 Monoclonal antibody (66634-1-Ig)

    WB analysis of rabbit serum using 66634-1-Ig (same clone as 66634-1-PBS)

    rabbit serum were subjected to SDS PAGE followed by western blot with 66634-1-Ig (C5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66634-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using C5 Monoclonal antibody (66634-1-Ig)

    IF Staining of HepG2 using 66634-1-Ig (same clone as 66634-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using C5 antibody (66634-1-Ig, Clone: 2G8C9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66634-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using C5 Monoclonal antibody (66634-1-Ig)

    IF Staining of HepG2 using 66634-1-Ig (same clone as 66634-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using C5 antibody (66634-1-Ig, Clone: 2G8C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66634-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using C5 Monoclonal antibody (66634-1-Ig)

    FC experiment of HepG2 using 66634-1-Ig (same clone as 66634-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human C5 (66634-1-Ig, Clone:2G8C9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66634-1-PBS in a different storage buffer formulation.

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C5 Monoclonal antibody, PBS Only

C5 Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 66634-1-PBS
Clone No.2G8C9

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

C5, C5b, C5, C5a, Complement C5, C5a anaphylatoxin, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66634-1-PBS targets C5 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen C5 fusion protein Ag17882 相同性解析による交差性が予測される生物種
Full Name complement component 5
Calculated molecular weight 1676 aa, 188 kDa
Observed molecular weight 115 kDa
GenBank accession numberBC113740
Gene Symbol C5
Gene ID (NCBI) 727
RRIDAB_2881993
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP01031
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The complement system is an important effector that bridges the innate and adaptive immune systems. The fifth component of complement, C5, is part of the complement cascade and plays an important role in inflammation and cell killing. It consists of disulfide-linked alpha and beta polypeptide chains but can be cleaved into two active peptides (C5a and C5b) by C5 convertases. Mutations and defects in C5 are associated with severe recurrent infections. (PMID: 20010915; PMID: 8011297; PMID: 6554279)