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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

C5AR1/CD88 Recombinant antibody

C5AR1/CD88 Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241712G8

Cat no : 84336-6-RR

Synonyms

C5AR, C5AR1, C5a-R, C5a R, C5a anaphylatoxin chemotactic receptor 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C5AR1/CD88 Recombinant antibody (84336-6-RR)

    WB analysis using 84336-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84336-6-RR (C5AR1/CD88 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using C5AR1/CD88 Recombinant antibody (84336-6-RR)

    IF Staining of THP-1 using 84336-6-RR

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using C5AR1/CD88 antibody (84336-6-RR, Clone: 241712G8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive WB detected inU-937 cells, U-87 MG cells
Positive IF/ICC detected inTHP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84336-6-RR targets C5AR1/CD88 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FusionProtein 相同性解析による交差性が予測される生物種
Full Name complement component 5a receptor 1
Calculated molecular weight39kDa
GenBank accession numberNM_001736.4
Gene symbol C5aR
Gene ID (NCBI) 728
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

C5aR, also named CD88, is a G protein-coupled receptor for the anaphylatoxin C5a, a cleavage product of the complement cascade. The C5a ligand is a proinflammatory component of host defense. C5aR is activated upon binding of the C5a molecule. Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release, and superoxide anion production. The deduced sequence of C5aR contains 350 amino acids, giving a calculated molecular weight of 39 kDa. C5aR has an N-linked glycosylation site in the N-terminal extracellular domain. The apparent molecular weight of C5aR is about 40-52 kDa (PMID: 1847994; 9136907; 9136907; 2007135).

Protocols

Product Specific Protocols
WB protocol for C5AR1/CD88 antibody 84336-6-RRDownload protocol
IF protocol for C5AR1/CD88 antibody 84336-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols