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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C5orf51 Polyclonal antibody (30107-1-AP)

    WB analysis using 30107-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30107-1-AP (C5orf51 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using C5orf51 Polyclonal antibody (30107-1-AP)

    WB analysis using 30107-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30107-1-AP (C5orf51 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using C5orf51 Polyclonal antibody (30107-1-AP)

    IHC staining of mouse liver using 30107-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30107-1-AP (C5orf51 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using C5orf51 Polyclonal antibody (30107-1-AP)

    IHC staining of mouse liver using 30107-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30107-1-AP (C5orf51 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using C5orf51 Polyclonal antibody (30107-1-AP)

    IF Staining of HeLa using 30107-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using C5orf51 antibody (30107-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using C5orf51 Polyclonal antibody (30107-1-AP)

    IF Staining of HeLa using 30107-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using C5orf51 antibody (30107-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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C5orf51 Polyclonal antibody

C5orf51 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 30107-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

C5orf51, UPF0600 protein C5orf51

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293T cells, HepG2 cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30107-1-AP targets C5orf51 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C5orf51 fusion protein Ag32440 相同性解析による交差性が予測される生物種
Full Name chromosome 5 open reading frame 51
Calculated molecular weight34kd
Observed molecular weight34-40 kDa
GenBank accession numberXM_011514032
Gene Symbol C5orf51
Gene ID (NCBI) 285636
RRIDAB_3086230
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDA6NDU8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for C5orf51 antibody 30107-1-APDownload protocol
IHC protocol for C5orf51 antibody 30107-1-APDownload protocol
IF protocol for C5orf51 antibody 30107-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols