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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C6orf108 Polyclonal antibody (25596-1-AP)

    WB analysis using 25596-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25596-1-AP (C6orf108 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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C6orf108 Polyclonal antibody

C6orf108 Polyclonal Antibody for WB, ELISA
Cat No. 25596-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

2'-deoxynucleoside 5'-phosphate N-hydrolase 1, c Myc responsive protein Rcl, C6orf108, DNPH1, RCL, RP3 330M21.3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inK-562 cells, Jurkat cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25596-1-AP targets C6orf108 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C6orf108 fusion protein Ag22336 相同性解析による交差性が予測される生物種
Full Name chromosome 6 open reading frame 108
Calculated molecular weight 174 aa, 19 kDa
Observed molecular weight 23 kDa
GenBank accession numberBC011683
Gene Symbol C6orf108
Gene ID (NCBI) 10591
RRIDAB_2880150
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43598
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

C6orf108 also named as DNPH1, catalyzes the cleavage of the N-glycosidic bond of deoxyribonucleoside 5'-monophosphates to yield deoxyribose 5-phosphate and a purine or pyrimidine base. Deoxyribonucleoside 5'-monophosphates containing purine bases are preferred to those containing pyrimidine bases (PMID: 24260472). Expressed at low levels in brain, colon, lung, peripheral blood leukocytes, placenta, small intestine, and thymus. Expressed at high levels in heart, kidney, liver, skeletal muscle and spleen. Overexpressed in a significant proportion of breast cancers (PMID: 18726892).

Protocols

Product Specific Protocols
WB protocol for C6orf108 antibody 25596-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols