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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C6orf108 Monoclonal antibody (68310-1-Ig)

    WB analysis using 68310-1-Ig (same clone as 68310-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68310-1-Ig (C6orf108 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68310-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using C6orf108 Monoclonal antibody (68310-1-Ig)

    IF Staining of HEK-293 using 68310-1-Ig (same clone as 68310-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using C6orf108 antibody (68310-1-Ig, Clone: 2C4E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 68310-1-PBS in a different storage buffer formulation.

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C6orf108 Monoclonal antibody, PBS Only

C6orf108 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68310-1-PBS
Clone No.2C4E1

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

DNPH1, EC:3.2.2.-, c-Myc-responsive protein RCL, c Myc responsive protein Rcl, 5-hydroxymethyl-dUMP N-hydrolase

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68310-1-PBS targets C6orf108 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen C6orf108 fusion protein Ag22382 相同性解析による交差性が予測される生物種
Full Name chromosome 6 open reading frame 108
Calculated molecular weight 174 aa, 19 kDa
Observed molecular weight23 kDa
GenBank accession numberBC011683
Gene Symbol C6orf108
Gene ID (NCBI) 10591
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO43598
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

C6orf108 also named as DNPH1, catalyzes the cleavage of the N-glycosidic bond of deoxyribonucleoside 5'-monophosphates to yield deoxyribose 5-phosphate and a purine or pyrimidine base. Deoxyribonucleoside 5'-monophosphates containing purine bases are preferred to those containing pyrimidine bases (PMID: 24260472). Expressed at low levels in brain, colon, lung, peripheral blood leukocytes, placenta, small intestine, and thymus. Expressed at high levels in heart, kidney, liver, skeletal muscle and spleen. Overexpressed in a significant proportion of breast cancers (PMID: 18726892).