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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

C6orf130 Recombinant antibody

C6orf130 Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240455D7

Cat no : 83500-4-RR

Synonyms

OARD1, O-acetyl-ADP-ribose deacetylase 1, EC:3.5.1.-, EC:3.2.2.-, ADP-ribose glycohydrolase OARD1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C6orf130 Recombinant antibody (83500-4-RR)

    WB analysis using 83500-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83500-4-RR (C6orf130 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of A431 cells using C6orf130 Recombinant antibody (83500-4-RR)

    FC experiment of A431 using 83500-4-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug C6orf130 Recombinant antibody (83500-4-RR, Clone:240455D7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83500-4-RR

    Affinity and Kinetic Characterization of 83500-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83500-4-RR against Human C6orf130 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HCT 116 cells
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83500-4-RR is C6orf130 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen C6orf130 fusion protein Ag19341 相同性解析による交差性が予測される生物種
Full Name chromosome 6 open reading frame 130
Calculated molecular weight 152 aa, 17 kDa
Observed molecular weight 17 kDa
GenBank accession numberBC011709
Gene symbol C6orf130
Gene ID (NCBI) 221443
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

OARD1 (O-acetyl-ADP-ribose deacetylase 1), also known as TARG1 (Terminal ADP-ribose protein glycohydrolase 1) and C6orf130, is a ADP-ribose glycohydrolase that hydrolyzes ADP-ribose and acts on different substrates, such as proteins ADP-ribosylated on glutamate and O-acetyl-ADP-D-ribose (PMID: 21849506; 23474714; 23481255). The MW of this protein is 17 kDa, and this antibody specially recognises the 17 kDa protein.

Protocols

Product Specific Protocols
WB protocol for C6orf130 antibody 83500-4-RRDownload protocol
FC protocol for C6orf130 antibody 83500-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols