Validation Data Gallery

Filter: AllWBIHCIF/ICCFC (Intra)

WB analysis using 28828-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 28828-1-AP (ATAT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 28828-1-AP
U-251 cells were subjected to SDS PAGE followed by western blot with 28828-1-AP (ATAT1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IHC staining of rat testis using 28828-1-AP
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 28828-1-AP (ATAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat testis using 28828-1-AP
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 28828-1-AP (ATAT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 28828-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28828-1-AP (ATAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 28828-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28828-1-AP (C6orf134 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of A549 using 28828-1-AP
Immunofluorescent analysis of (4% PFA) fixed A549 cells using ATAT1 antibody (28828-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of A549 using 28828-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using ATAT1 antibody (28828-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of A549 using 28828-1-AP
Immunofluorescent analysis of (4% PFA) fixed A549 cells using ATAT1 antibody (28828-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of A549 using 28828-1-AP
Immunofluorescent analysis of (4% PFA) fixed A549 cells using ATAT1 antibody (28828-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
FC experiment of A431 using 28828-1-AP
1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human ATAT1 (28828-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO Validated

ATAT1 Polyclonal antibody

ATAT1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 28828-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

C6orf134, Nbla00487, MEC17, FLJ13158, alpha-TAT1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (6)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A549 cells, U-251 cells, THP-1 cells, U-87 MG cells
Positive IHC detected in	rat testis tissue, mouse testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A549 cells
Positive FC (Intra) detected in	A431 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 6 publications below
IF	See 2 publications below

Product Information

28828-1-AP targets ATAT1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ATAT1 fusion protein Ag28757 相同性解析による交差性が予測される生物種
Full Name	chromosome 6 open reading frame 134
Calculated molecular weight	47 kDa
Observed molecular weight	42-45 kDa
GenBank accession number	NM_001031722
Gene Symbol	ATAT1
Gene ID (NCBI)	79969
RRID	AB_2881219
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q5SQI0
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

C6orf134 is a reported alias name for the human gene ATAT1 coding alpha-tubulin N-acetyltransferase 1, and specifically acetylates 'Lys-40' in alpha-tubulin on the lumenal side of microtubules. May affect microtubule stability and regulate microtubule dynamics. May be involved in neuron development. Western blot shows a band between 36 kDa and 45 kDa.

Protocols

Product Specific Protocols
WB protocol for ATAT1 antibody 28828-1-AP	Download protocol
IHC protocol for ATAT1 antibody 28828-1-AP	Download protocol
IF protocol for ATAT1 antibody 28828-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB,IF	iScience MFN2 coordinates mitochondria motility with α-tubulin acetylation and this regulation is disrupted in CMT2A Authors - Atul Kumar View Article
rat	WB	Biochem Biophys Res Commun NWD1 influences the extension of neuronal axons by regulating microtubule stability Authors - Tiancheng Bao View Article
human	WB	Cancer Cell Int Suppression of microtubule acetylation mediates the anti-leukemic effect of CDK9 inhibition Authors - Xi Xie KD Validated View Article
mouse	WB	Cell Death Dis TMEM232 is required for the formation of sperm flagellum and male fertility in mice Authors - Xinying Cai View Article
human	WB	MedComm (2020) Matrix stiffness-induced α-tubulin acetylation is required for skin fibrosis formation through activation of Yes-associated protein Authors - Dongsheng Wen KD Validated View Article
rat	WB,IF	Biochim Biophys Acta Mol Cell Res Eml1 promotes axonal growth by enhancing αTAT1-mediated microtubule acetylation Authors - Yufang Zhang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 28828-1-AP

WB analysis using 28828-1-AP

IHC staining of rat testis using 28828-1-AP

IHC staining of rat testis using 28828-1-AP

IHC staining of mouse testis using 28828-1-AP

IHC staining of mouse testis using 28828-1-AP

IF Staining of A549 using 28828-1-AP

IF Staining of A549 using 28828-1-AP

IF Staining of A549 using 28828-1-AP

IF Staining of A549 using 28828-1-AP

FC experiment of A431 using 28828-1-AP

ATAT1 Polyclonal antibody

ATAT1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

MFN2 coordinates mitochondria motility with α-tubulin acetylation and this regulation is disrupted in CMT2A

NWD1 influences the extension of neuronal axons by regulating microtubule stability

Suppression of microtubule acetylation mediates the anti-leukemic effect of CDK9 inhibition

TMEM232 is required for the formation of sperm flagellum and male fertility in mice

Matrix stiffness-induced α-tubulin acetylation is required for skin fibrosis formation through activation of Yes-associated protein

Eml1 promotes axonal growth by enhancing αTAT1-mediated microtubule acetylation