Validation Data Gallery
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Tested Applications
| Positive WB detected in | A375 cells |
| Positive IHC detected in | mouse skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A375 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 3 publications below |
| IF | See 1 publications below |
Product Information
24953-1-AP targets C6orf15 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | C6orf15 fusion protein Ag20276 相同性解析による交差性が予測される生物種 |
| Full Name | chromosome 6 open reading frame 15 |
| Calculated molecular weight | 325 aa, 34 kDa |
| Observed molecular weight | 50-55 kDa |
| GenBank accession number | BC153157 |
| Gene Symbol | C6orf15 |
| Gene ID (NCBI) | 29113 |
| RRID | AB_2879818 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6UXA7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
C6orf15, also named as Protein STG, is a 325 amino acid protein, which as a secreted protein is expressed in skin and tonsils. The calculated molecular weight of C6orf15 is 34 kDa, but we detected a 50-55 kDa protein by western blot. C6orf15 may contains some modified residues.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for C6orf15 antibody 24953-1-AP | Download protocol |
| IHC protocol for C6orf15 antibody 24953-1-AP | Download protocol |
| IF protocol for C6orf15 antibody 24953-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Cell Int C6orf15 promotes liver metastasis via WNT/β-catenin signalling in colorectal cancer | ||
Pathol Res Pract C6orf15 acts as a potential novel marker of adverse pathological features and prognosis for colon cancer | ||
Ann Diagn Pathol Differential diagnosis of gastric low- and high grade dysplasia using C6orf15 protein |