Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | A549 cells, mouse kidney tissue |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| IF | See 3 publications below |
Product Information
24957-1-AP targets C6orf182 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, monkey samples.
| Tested Reactivity | human, mouse, monkey |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | C6orf182 fusion protein Ag21054 相同性解析による交差性が予測される生物種 |
| Full Name | chromosome 6 open reading frame 182 |
| Calculated molecular weight | 460 aa, 54 kDa |
| Observed molecular weight | 55-65 kda |
| GenBank accession number | BC064365 |
| Gene Symbol | C6orf182 |
| Gene ID (NCBI) | 285753 |
| RRID | AB_2879821 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IYX8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
C6orf182 also named as Centrosomal protein of 57 kDa related protein is a 460 amino-acid protein, which belongs to translokin family. C6orf182 as centrosomal protein may be required for microtubule attachment to centrosomes and localizes in the cytoplasm. The function of C6orf182 need to be further studied.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for C6orf182 antibody 24957-1-AP | Download protocol |
| IF protocol for C6orf182 antibody 24957-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Cell Biol Cep57 and Cep57L1 maintain centriole engagement in interphase to ensure centriole duplication cycle. | ||