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Validation Data Gallery

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  • Western Blot (WB) analysis of human testis tissue using KHDC3L Polyclonal antibody (24989-1-AP)

    WB analysis of human testis using 24989-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 24989-1-AP (KHDC3L antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using KHDC3L Polyclonal antibody (24989-1-AP)

    IHC staining of human liver cancer using 24989-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24989-1-AP (KHDC3L antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using KHDC3L Polyclonal antibody (24989-1-AP)

    IHC staining of human liver cancer using 24989-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24989-1-AP (KHDC3L antibody at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using KHDC3L Polyclonal antibody (24989-1-AP)

    IF Staining of HepG2 using 24989-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using KHDC3L antibody (24989-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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KHDC3L Polyclonal antibody

KHDC3L Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 24989-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

C6orf221, Filia, KHDC3-like protein, KH domain-containing protein 3, ES cell-associated transcript 1 protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration:  150 μL, 1000 ug/ml
20 μL, 1000 ug/ml
150 μL, 1000 ug/ml

¥67,000/ 150 μL

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman testis tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24989-1-AP targets KHDC3L in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KHDC3L fusion protein Ag21790 相同性解析による交差性が予測される生物種
Full Name chromosome 6 open reading frame 221
Calculated molecular weight 217 aa, 24 kDa
Observed molecular weight 21-24 kDa
GenBank accession numberBC137160
Gene Symbol KHDC3L
Gene ID (NCBI) 154288
RRIDAB_2879833
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ587J8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KHDC3L (also known as Filia and ES cell-associated transcript1 [Ecat1]) as a key regulator in safeguarding the genomic stability of mouse early embryonic cells. KHDC3L is critical in preventing the replication associated DNA DSBs in epiblast cells (PMID: 29125140). Importantly, by analyzing KHDC3L mutations causes recurrent pregnancy loss by inducing genomic instability of human early embryonic cells (PMID: 31609975).

Protocols

Product Specific Protocols
WB protocol for KHDC3L antibody 24989-1-APDownload protocol
IHC protocol for KHDC3L antibody 24989-1-APDownload protocol
IF protocol for KHDC3L antibody 24989-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols