C7orf10 Polyclonal antibody

Name: C7orf10 Polyclonal antibody
Brand: Proteintech
SKU: 21589-1-AP

C7orf10 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 21589-1-AP

Synonyms

C7orf10, DERP13, ORF19

Validation Data Gallery

Filter:

AllWBIHC

WB analysis using 21589-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21589-1-AP (C7orf10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 21589-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 21589-1-AP (C7orf10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 21589-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21589-1-AP (C7orf10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 21589-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 21589-1-AP (C7orf10 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse pancreas using 21589-1-AP
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 21589-1-AP (C7orf10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human kidney using 21589-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 21589-1-AP (C7orf10 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 21589-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 21589-1-AP (C7orf10 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human pancreas using 21589-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 21589-1-AP (C7orf10 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human pancreas using 21589-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 21589-1-AP (C7orf10 antibody) at dilution of 1:50 (under 40x lens).

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Cat No. 21589-1-AP

Size/Concentration

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse kidney tissue, mouse pancreas tissue, mouse skeletal muscle tissue, HepG2 cells, HEK-293 cells, rat kidney tissue
Positive IHC detected in	human kidney tissue, human pancreas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below

Product Information

21589-1-AP targets C7orf10 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	C7orf10 fusion protein Ag16159 相同性解析による交差性が予測される生物種
Full Name	chromosome 7 open reading frame 10
Calculated molecular weight	471 aa, 51 kDa
Observed molecular weight	48-55 kDa
GenBank accession number	BC098318
Gene symbol	C7orf10
Gene ID (NCBI)	79783
RRID	AB_10734435
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

C7orf10 is also named as SUGCT, DERP13 and belongs to the CaiB/BaiF CoA-transferase family. It may function as part of a multiunit enzyme complex (A), similar to the alpha-ketoglutarate dehydrogenase system, or as the second of two independent enzymes, the first of which decarboxylates alpha-ketoadipate to glutarate (B). This protein has 4 isoforms produced by alternative splicing with the molecular weight from 43 kDa to 51 kDa.

Protocols

Product Specific Protocols
WB protocol for C7orf10 antibody 21589-1-AP	Download protocol
IHC protocol for C7orf10 antibody 21589-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Cell Mol Life Sci Knockout of the non-essential gene SUGCT creates diet-linked, age-related microbiome disbalance with a diabetes-like metabolic syndrome phenotype. Authors - Joanna Niska-Blakie View Article
human	WB	Front Aging Neurosci A Y374X TDP43 truncation leads to an altered metabolic profile in amyotrophic lateral sclerosis fibroblasts driven by pyruvate and TCA cycle intermediate alterations Authors - Scott P Allen View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

C7orf10 Polyclonal antibody

C7orf10 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis using 21589-1-AP

WB analysis of HepG2 using 21589-1-AP

WB analysis of mouse skeletal muscle using 21589-1-AP

WB analysis of HEK-293 using 21589-1-AP

WB analysis of mouse pancreas using 21589-1-AP

IHC staining of human kidney using 21589-1-AP