C7orf30 Polyclonal antibody

Name: C7orf30 Polyclonal antibody
Brand: Proteintech
SKU: 22838-1-AP

C7orf30 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 22838-1-AP

Synonyms

C7orf30, MALSU1

Validation Data Gallery

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AllWBIHC

WB analysis using 22838-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22838-1-AP (C7orf30 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 22838-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 22838-1-AP (C7orf30 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human testis using 22838-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 22838-1-AP (C7orf30 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 22838-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 22838-1-AP (C7orf30 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human liver using 22838-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 22838-1-AP (C7orf30 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver using 22838-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 22838-1-AP (C7orf30 antibody) at dilution of 1:50 (under 40x lens).

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Cat No. 22838-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, HepG2 cells, K-562 cells, U-251 cells
Positive IHC detected in	human testis tissue, human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 5 publications below

Product Information

22838-1-AP targets C7orf30 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	C7orf30 fusion protein Ag18286 相同性解析による交差性が予測される生物種
Full Name	chromosome 7 open reading frame 30
Calculated molecular weight	234 aa, 26 kDa
Observed molecular weight	26-28 kDa
GenBank accession number	BC012331
Gene symbol	C7orf30
Gene ID (NCBI)	115416
RRID	AB_11182483
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

C7orf30, also known as MALSU1, is a member of the iojap protein family. It was predicted to enable ribosomal large subunit binding activity. It was Involved in negative regulation of mitochondrial translation and ribosomal large subunit biogenesis.

Protocols

Product Specific Protocols
WB protocol for C7orf30 antibody 22838-1-AP	Download protocol
IHC protocol for C7orf30 antibody 22838-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Commun Structural basis of GTPase-mediated mitochondrial ribosome biogenesis and recycling. Authors - Hauke S Hillen View Article
human	WB	Nucleic Acids Res The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis. Authors - Elena Lavdovskaia View Article
human	WB	Cell Rep The Human Mitochondrial DEAD-Box Protein DDX28 Resides in RNA Granules and Functions in Mitoribosome Assembly. Authors - Ya-Ting Tu View Article
human	WB	bioRxiv Tetracycline-dependent inhibition of mitoribosome protein elongation in mitochondrial disease mutant cells suppresses IRE1α to promote cell survival Authors - Conor T Ronayne View Article
human	WB	EMBO Rep Tetracyclines activate mitoribosome quality control and reduce ER stress to promote cell survival Authors - Conor T Ronayne View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

C7orf30 Polyclonal antibody

C7orf30 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis using 22838-1-AP

WB analysis of HeLa using 22838-1-AP

IHC staining of human testis using 22838-1-AP

IHC staining of human testis using 22838-1-AP

IHC staining of human liver using 22838-1-AP

IHC staining of human liver using 22838-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Structural basis of GTPase-mediated mitochondrial ribosome biogenesis and recycling.

The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.

The Human Mitochondrial DEAD-Box Protein DDX28 Resides in RNA Granules and Functions in Mitoribosome Assembly.

Tetracycline-dependent inhibition of mitoribosome protein elongation in mitochondrial disease mutant cells suppresses IRE1α to promote cell survival

Tetracyclines activate mitoribosome quality control and reduce ER stress to promote cell survival