C7orf50 Polyclonal antibody
C7orf50 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 20797-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293T cells, Jurkat cells, L02 cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | L02 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
20797-1-AP targets C7orf50 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | C7orf50 fusion protein Ag14709 相同性解析による交差性が予測される生物種 |
| Full Name | chromosome 7 open reading frame 50 |
| Calculated molecular weight | 194 aa, 22 kDa |
| Observed molecular weight | 22 kDa |
| GenBank accession number | BC006224 |
| Gene symbol | C7orf50 |
| Gene ID (NCBI) | 84310 |
| RRID | AB_10732839 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
C7orf50 (Chromosome 7, Open Reading Frame 50) is ubiquitously expressed in the kidneys, brain, fat, prostate, spleen, among 22 other tissues and demonstrates low tissue specificity.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for C7orf50 antibody 20797-1-AP | Download protocol |
| IHC protocol for C7orf50 antibody 20797-1-AP | Download protocol |
| IF protocol for C7orf50 antibody 20797-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Phosphorylation of the Ribosomal Protein RPL12/uL11 Affects Translation during Mitosis. | ||
