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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HIG2 Polyclonal antibody (15587-1-AP)

    WB analysis using 15587-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15587-1-AP (HIG2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using HIG2 Polyclonal antibody (15587-1-AP)

    IF Staining of A549 using 15587-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using HIG2 antibody (15587-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

HIG2 Polyclonal antibody

HIG2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 15587-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

C7orf68, Hypoxia-inducible lipid droplet-associated protein, Hypoxia-inducible gene 2 protein, HILPDA, HIG-2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15587-1-AP targets HIG2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HIG2 fusion protein Ag7829 相同性解析による交差性が予測される生物種
Full Name chromosome 7 open reading frame 68
Calculated molecular weight 7 kDa
Observed molecular weight 7-10 kDa
GenBank accession numberBC001863
Gene Symbol C7orf68
Gene ID (NCBI) 29923
RRIDAB_3669213
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y5L2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HIG2 (Hypoxia inducible gene 2), also known as hypoxia inducible lipid droplet associated (HILPDA), is a gene that plays a significant role in the context of hypoxia, particularly in cancer biology. HIG2 is can be induced by hypoxia and glucose deprivation. It is expressed under low-oxygen conditions, which are common in the tumor microenvironment, and has been identified as a target gene of hypoxia-inducible factor-1 (HIF-1). HIG2 is implicated in the development and progression of various types of cancer, including renal cell carcinoma(PMID: 15930302), cell lymphoma(PMID: 26757780), epithelial ovarian cancer(PMID: 20134266), and uterine cancer(PMID: 21614900). Its expression is often elevated in these cancers and is associated with poor prognosis.

Protocols

Product Specific Protocols
WB protocol for HIG2 antibody 15587-1-APDownload protocol
IF protocol for HIG2 antibody 15587-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols