Validation Data Gallery
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Tested Applications
| Positive WB detected in | human plasma |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25289-1-AP targets C8A in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | C8A fusion protein Ag18389 相同性解析による交差性が予測される生物種 |
| Full Name | complement component 8, alpha polypeptide |
| Calculated molecular weight | 584 aa, 65 kDa |
| Observed molecular weight | 65-70 kDa |
| GenBank accession number | BC132913 |
| Gene Symbol | C8A |
| Gene ID (NCBI) | 731 |
| RRID | AB_3085781 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P07357 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
C8 is a component of the complement system and contains three polypeptides, alpha, beta and gamma. Complement component C8 alpha chain (C8A) is a constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C8A inserts into the target membrane, but does not form pores by itself. Mutations in the C8A gene cause complement C8 alpha-gamma deficiency.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for C8A antibody 25289-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |