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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using AFAF/EQTN Polyclonal antibody (23168-1-AP)

    WB analysis of HepG2 using 23168-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 23168-1-AP (AFAF/EQTN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using AFAF/EQTN Polyclonal antibody (23168-1-AP)

    WB analysis of HeLa using 23168-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 23168-1-AP (AFAF/EQTN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using AFAF/EQTN Polyclonal antibody (23168-1-AP)

    IF Staining of HepG2 using 23168-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using AFAF/EQTN antibody (23168-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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AFAF/EQTN Polyclonal antibody

AFAF/EQTN Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 23168-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

AFAF, C9orf11, EQTN, Equatorin

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, HeLa cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23168-1-AP targets AFAF/EQTN in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AFAF/EQTN fusion protein Ag19656 相同性解析による交差性が予測される生物種
Full Name chromosome 9 open reading frame 11
Calculated molecular weight 294 aa, 33 kDa
Observed molecular weight 27-29 kDa
GenBank accession numberBC137351
Gene Symbol AFAF
Gene ID (NCBI) 54586
RRIDAB_2879222
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NQ60
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The AFAF gene (also known as EQTN and C9orf11) encodes a membrane protein that is specifically localized to the acrosomal membrane. AFAF is involved in the process of fertilization and acrosome biogenesis. Expression analysis demonstrates that AFAF is highly expressed in the testis, although minor expression was seen in other tissues. AFAF has 3 isoforms with molecular weights of 14, 29, and 33 kDa. However, one article detected molecular weight between 40-65 kDa in mouse testis (PMID: 19605790).

Protocols

Product Specific Protocols
WB protocol for AFAF/EQTN antibody 23168-1-APDownload protocol
IF protocol for AFAF/EQTN antibody 23168-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols