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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

C9orf117 Polyclonal antibody

C9orf117 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21012-1-AP

Synonyms

Equatorin, EQTN, Cilia- and flagella-associated protein 157, AFAF

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C9orf117 Polyclonal antibody (21012-1-AP)

    WB analysis using 21012-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21012-1-AP (C9orf117 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using C9orf117 Polyclonal antibody (21012-1-AP)

    IHC staining of mouse brain using 21012-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21012-1-AP (C9orf117 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using C9orf117 Polyclonal antibody (21012-1-AP)

    IHC staining of mouse brain using 21012-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21012-1-AP (C9orf117 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inCOLO 320 cells, Raji cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21012-1-AP targets C9orf117 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C9orf117 fusion protein Ag15234 相同性解析による交差性が予測される生物種
Full Name chromosome 9 open reading frame 117
Calculated molecular weight 520 aa, 61 kDa
Observed molecular weight60 kDa
GenBank accession numberBC141809
Gene symbol C9orf117
Gene ID (NCBI) 286207
RRIDAB_3669353
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

C9orf117 (Cilia- and flagella-associated protein 157, CFAP157) protein localizes to basal bodies and interacts with tubulin and the centrosomal protein CEP350 (PMID: 27965440).

Protocols

Product Specific Protocols
WB protocol for C9orf117 antibody 21012-1-APDownload protocol
IHC protocol for C9orf117 antibody 21012-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols