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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NGX6 Polyclonal antibody

NGX6 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 21593-1-AP

Synonyms

TMEM8B, C9orf127, Transmembrane protein 8B, Protein NAG-5, Nasopharyngeal carcinoma-associated gene 6 protein

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of rat small intestine tissue using NGX6 Polyclonal antibody (21593-1-AP)

    IHC staining of rat small intestine using 21593-1-AP

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 21593-1-AP (NGX6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat stomach tissue using NGX6 Polyclonal antibody (21593-1-AP)

    IHC staining of rat stomach using 21593-1-AP

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 21593-1-AP (NGX6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using NGX6 Polyclonal antibody (21593-1-AP)

    IHC staining of mouse stomach using 21593-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 21593-1-AP (NGX6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using NGX6 Polyclonal antibody (21593-1-AP)

    IHC staining of mouse testis using 21593-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21593-1-AP (NGX6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse intestine using NGX6 Polyclonal antibody (21593-1-AP)

    IHC staining of mouse intestine using 21593-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse intestine tissue slide using 21593-1-AP (NGX6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat testis tissue using NGX6 Polyclonal antibody (21593-1-AP)

    IHC staining of rat testis using 21593-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 21593-1-AP (NGX6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using NGX6 Polyclonal antibody (21593-1-AP)

    IHC staining of human stomach cancer using 21593-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21593-1-AP (NGX6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using NGX6 Polyclonal antibody (21593-1-AP)

    IHC staining of human cervical cancer using 21593-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21593-1-AP (NGX6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using NGX6 Polyclonal antibody (21593-1-AP)

    IHC staining of human colon cancer using 21593-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21593-1-AP (NGX6 antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using NGX6 Polyclonal antibody (21593-1-AP)

    IHC staining of human colon cancer using 21593-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21593-1-AP (NGX6 antibody at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive IHC detected inhuman stomach cancer tissue, human colon cancer tissue, mouse intestine, rat small intestine tissue, rat stomach tissue, mouse stomach tissue, mouse testis tissue, human cervical cancer tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21593-1-AP targets NGX6 in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NGX6 fusion protein Ag16167 相同性解析による交差性が予測される生物種
Full Name chromosome 9 open reading frame 127
Calculated molecular weight 472 aa, 52 kDa
GenBank accession numberBC098296
Gene symbol NGX6
Gene ID (NCBI) 51754
RRIDAB_2878889
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NGX6 (also known as TMEM8B or C9orf127) is a transmembrane protein containing an epidermal growth factor (EGF)-like domain. It may function as a regulator of the EGFR pathway. NGX6 has been reported as a tumor suppressor which may function in cell growth, proliferation and adhesion (PMID: 19755717; 12918109; 20705583).

Protocols

Product Specific Protocols
IHC protocol for NGX6 antibody 21593-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols